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The optimum pH was determined by performing enzymatic assays at different pH levels (4.7 to 9.76) at 37C

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To corroborate these findings on a functional level, we isolated activated ILC2s from IL-33 challenged BALB/c mice ( Figure 3A ), which were then incubated for 24h with either an MT1 agonist (Ramelteon), MT1 antagonist (S-26131), MT2 agonist (Tasimelteon), MT2 antagonist (4-P-PDOT) and/or melatonin ( Figures 3BE )